Fig. 4

Acetate regulation of microglia morphology in the RVLM of SHRs. A, illustrative figures showcasing the expression of ionized calcium-binding adapter molecule 1 (IBA1) in the RVLM (top panes), along with photomicrographs of distinct IBA1-positive microglia (middle panes), accompanied by their respective traced contours in the lower panels (bottom panes). B-F, quantification of the density, soma size, number of branches, the maximal and average branches length of IBA1⁺ microglia. G, a non-linear curve fitting represents the average count of microglial branch intersections per 10 μm increment away from the cell body, as determined through Sholl analysis. H, the total area under the curve (AUC) of individual microglia, derived from Sholl analysis. n = 5 for each group. * P < 0.05, ** P < 0.01, *** P < 0.001