Fig. 10

IBA1+ cells in the PD CNS of EAE mice have activated morphology and low complexity. CNS sections of CD and PD mice isolated during EAE onset were immunostained for IBA1. A/D Representative images of IBA1 immunoreactivity in lumbar spinal cords (A) and cerebellar (D) tissues of mice. B/E. Quantification of IBA1 MFI of lumbar spinal cord (B) and cerebellar (E) tissues of CD and PD mice. C/F Sholl analysis of IBA1+ cells near inflammatory foci shows low complexity and activated morphology in PD mice, but increased complexity in CD mice. (C: spinal cord; F cerebellum). Scale bars denote 100 μm in whole tissue images and 25 μm in enlarged cellular-level images. Data are shown as means ± SD, n = 6–7 mice; 2 sections per tissue; 4–8 microglia per section; *p < 0.05