Fig. 12

Increased demyelination in the cerebellum and decreased demyelination in the spinal cord of PD mice during EAE onset. Myelin was detected in lumbar spinal cord and cerebellar tissues isolated from mice during EAE onset via MBP immunostaining. Demyelinated areas (i.e., white matter devoid of MBP immunoreactivity; demarcated with small white borders) are readily detected in the spinal cords of CD but not in the spinal cords of PD (A) and cerebella of PD mice but not cerebella of CD mice (B). Quantification shows percentage of MBP devoid white matter (WM) with all WM area (graphs on the right). Scale bars denote 100 μm. Data are shown as means ± SD, n = 7; at least 2 sections per tissue; *p < 0.05, **p < 0.005