Fig. 2From: CSF1R antagonism results in increased supraspinal infiltration in EAEEffect of PLX5622 on myeloid cell subsets in peripheral immune tissues in steady state. Flow cytometric analysis of single cells isolated from spleen, bone marrow, and skin in CD and PD mice. Neutrophils (CD45+CD11b+Ly6G+), monocytes (monos; CD45+CD11b+CD11c-Ly6G-MHCII-Ly6C+), macrophages (macros; CD45+CD11b+CD11c-Ly6G-MHCII+) and dendritic cells (DCs; CD45+CD11b+CD11c+MHCII+) were analyzed in A spleen and B bone marrow. Monocytes and macrophage subsets were further classified as Ly6C+ (inflammatory) or Ly6C- (non-inflammatory). C In skin, dendritic cells (CD45+CD11b+CD11c+MHCII+), langerin + cells (CD45+CD11b+Ly6G-CD11c+MHCII+langerin+; includes Langerhans cells in the epidermis and langerin+ DCs in the dermis), and monocyte/macrophage populations were analyzed. Monocytes and macrophage subsets were further classified as Ly6C+ or Ly6C-. Representative flow plots are shown. Both male and female mice were employed. Results are shown as means ± SD, n = 6–7; *p < 0.05, **p < 0.005, ****p < 0.00005Back to article page