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Table 1 Descriptive statistics, two-factor ANOVA, and test of simple effects analysis for immune markers after immunophenotyping with flow cytometry

From: The immunomodulatory effect of oral NaHCO3 is mediated by the splenic nerve: multivariate impact revealed by artificial neural networks

 

CD11bc + CD38 + cells

M1-like macrophage

M2-like macrophage

Granularity index in FSChigh cells

Macrophage polarization ratio

T-helper

Treg

CD4-T cells

CD4-FoxP3 + T cells

Treg/M1-like

 

CD11bc + CD38 + 

Log10 CD11bc + CD38 + TNFα + 

CD11bc + CD206 + 

CD11bc + SSChigh/SSClow

Log10 M1-like/M2-like

Log10 CD3 + CD4 + 

CD3 + CD4 + FoxP3 + 

CD3 + CD4−FoxP3−

CD3 + CD4−FoxP3 + 

Log10 Treg/M1-like

Descriptive statistics

          

 SH H2O (n = 11)

68.44 ± 5.29

1.33 ± 0.23

1.82 ± 0.74

5.22 ± 2.23

1.09 ± 0.27

2.72 ± 0.36

10.18 ± 1.44

42.61 ± 6.36

1.01 ± 0.31

1.67 ± 0.229

 SH NaHCO3 (n = 13)

67.43 ± 6.62

1.15 ± 0.15

2.23 ± 0.43

2.87 ± 0.97

0.82 ± 0.17

2.72 ± 0.45

11.79 ± 1.86

41.85 ± 8.19

1.06 ± 0.35

1.92 ± 0.15

 SD H2O (n = 7)

74.49 ± 8.16

1.18 ± 0.22

1.89 ± 0.49

3.70 ± 2.17

0.92 ± 0.26

2.68 ± 0.40

8.22 ± 2.84

46.94 ± 5.54

0.70 ± 0.37

1.71 ± 0.28

 SD NaHCO3 (n = 9)

70.85 ± 8.33

1.18 ± 0.17

1.53 ± 0.55

3.63 ± 1.39

1.02 ± 0.25

2.71 ± 0.68

6.83 ± 2.61

44.72 ± 10.22

0.71 ± 0.49

1.63 ± 0.21

 Levene’s Test, p

0.430

0.607

0.125

0.073

0.443

0.083

0.088

0.177

0.350

0.305

 Distribution (KS), p

0.200

0.200

0.200

0.200

0.146

0.200

0.132

0.172

0.200

0.200

Two-factor ANOVA

          

 Interaction (Tr*Sx) p

0.559

0.137

0.005**

0.070

0.018*

0.690

0.038*

0.777

0.861

0.026*

 pes

0.010

0.058

0.202**

0.088*

0.147**

0.004

0.115*

0.002

0.001

0.131*

 (Tr) F

1.031

    

0.233

 

0.339

0.047

 

 p

0.317

    

0.632

 

0.564

0.830

 

 pes

0.028

    

0.006

 

0.009

0.001

 

 (Sx) F

4.400

    

0.446

 

1.980

7.160

 

 p

0.043*

    

0.509

 

0.168

0.011*

 

 pes

0.109*

    

0.012

 

0.052

0.166**

 

Test of simple effects

          

 (Tr) SH H2OxNaHCO3, F

 

5.245

6.645

9.388

8.238

 

3.366

  

7.758

 p

 

0.028*

0.014*

0.004**

0.007**

 

0.075

  

0.009**

 pes

 

0.127*

0.156**

0.207**

0.186**

 

0.086*

  

0.174**

 (Tr) SD H2OxNaHCO3, F

 

0.006

3.224

0.008

0.765

 

1.669

  

0.583

 p

 

0.941

0.081

0.930

0.387

 

0.205

  

0.450

 pes

 

0.000

0.082*

0.000

0.021

 

0.044

  

0.016

( Sx) H2O SHxSD F

 

2.384

0.798

3.124

2.491

 

3.565

  

0.095

 p

 

0.131

0.378

0.086

0.123

 

0.067

  

0.760

 pes

 

0.062 *

0.022

0.080*

0.065*

 

0.090*

  

0.003

 (Sx) NaHCO3 SHxSD F

 

0.126

12.431

0.675

3.876

 

28.519

  

9.871

 p

 

0.725

0.001**

0.417

0.057

 

< 0.001**

  

0.003**

 pes

 

0.003

0.257**

0.018

0.097*

 

0.442**

  

0.215**

  1. SH, animals with sham surgery; SD, animals with spleen denervation; Tr, treatment either H2O or NaHCO3; Sx, surgery either SD or SH; KS, Kolmogorov Smirnov; p, probability (significant for p < 0.05 except for the variables with an interaction between treatment and surgery where p < 0.15); pes, partial eta squared (small = pes < 0.06, medium = 0.06 < pes < 0.14, large = pes > 0.14); Tr, factor treatment which included either H2O or NaHCO3; Sx, factor surgery which included; *significant differences p < 0.05 or moderate size effect (pse > 0.06); **p < 0.01 or large SE (pse > 0.14)
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Journal of Neuroinflammation

ISSN: 1742-2094