Fig. 1
From: Impact of microglia isolation and culture methodology on transcriptional profile and function
Overview of microglial isolation protocols. A Protocol 1 involves manually shaking microglia from MGCs sourced from p0-p3 pups with 3 variations: (1A) 10-day culture with 0.5 ng/mL GM-CSF, (1B) 10-day culture without GM-CSF, and (1C) 21-day culture with 0.5 ng/mL GM-CSF. B Protocol 2 involves microglia isolated by adhesive properties from a whole brain suspension, by replacing media after 3 h, then cultured for 7 days with 100 ng/mL GM-CSF and M-CSF. C Protocol 3 involves adult microglia isolated using anti-CD11b magnetic beads, and then immediately used for experimentation. Created with Biorender.com