Fig. 3
From: Impact of microglia isolation and culture methodology on transcriptional profile and function
Differential cytokine secretion and transcription in response to HMGB1 stimulation in microglia derived from isolation protocols. A–E Cytokine secretion by microglia, quantified as concentration interpolated from a standard curve, 24 h post-HMGB1 stimulation. For each panel, grouped bar graphs represent concentration of TNFα, IL1β, and IFNβ in culture supernatant. F–J Transcription of the same cytokines as determined by bulk mRNA sequencing, displayed as normalized gene counts derived using DESeq2. Grouped bar graphs indicating transcription of TNFα, IL1β, and IFNβ. A, F Microglia derived from Protocol 1A. B, G Microglia derived from Protocol 1B. C, H Microglia derived from Protocol 1C. D, I Microglia derived from Protocol 2. E, J Microglia derived from Protocol 3. Dots represent microglia isolations derived from distinct litters of mice for neonatal mice or distinct adult mice