Fig. 4
From: Impact of microglia isolation and culture methodology on transcriptional profile and function
Effects of HMGB1 on transcriptomic profile of microglia from each protocol. A–C In a PCA scatter plot (A, B), microglial isolation protocols are represented by shapes: Circle (Protocol 1A), Square (1B), Diamond (1C), Triangle (2), and Upside-down triangle (3). Treatment colors are Red (Control) and Blue (HMGB1), with three replicates each. A Pup derived microglia are highlighted; B Adult derived microglia are highlighted. Grouped bar graphs (C) exhibit the number of DEGs after HMGB1 treatment across protocols, differentiated by Red (downregulated) and Green (upregulated) bars, with significance set at an adjusted p-value < 0.05. D–F Log2FoldChange plots display the top 250 genes, marking the 20 most significant. D compares Protocol 1A (X-axis) and 2 (Y-axis); E compares 1A (X-axis) and 3 (Y-axis); F compares 2 (X-axis) and 3 (Y-axis). Genes significant in the first protocol are Green, in the second are Blue, and in both are Red