Fig. 3

NSA or Mdivi-1 attenuated the activation of microglia and astrocytes and the release of inflammatory cytokines in the hippocampus of SAE mice. A Representative images of Iba-1 (green) in the hippocampal CA1 region. B Quantification of Iba-1 fluorescence hippocampal CA1 region. C Representative images of GFAP (red) in the hippocampal CA1 region. D Quantification of GFAP fluorescence hippocampal CA1 region. Data are presented as mean ± SEM (n = 4–6 mice/group). DAPI staining is shown in blue. Scale bar = 50 μm. E–G Representative western blotting and quantitative analysis of the protein levels of IL-1β, IL-18 and TNF-a in the hippocampus. Data are presented as the mean ± SEM (n = 4 mice/group). ^*P < 0.05, ^**P < 0.01 versus the indicated groups