Fig. 5

NSA or Mdivi-1 reduced neuronal damages in the hippocampus of SAE mice. A Representative drawing of HE staining showing morphologic appearance of neurons in the hippocampal CA1 region, the black arrows showed the marked nuclear pyknosis and necrotic neuron. Scale bar = 50 μm. B Semiquantitative analysis in the hippocampal neuronal damage scores. C Representative images of TUNEL staining (red) and NeuN (green) in the hippocampal CA1 region (the white arrows showed TUNEL-NeuN positive neurons). DAPI staining is shown in blue. Scale bar = 50 μm. D Numbers of TUNEL-NeuN positive neurons in the hippocampal CA1 region. Data are presented as the mean ± SEM (n = 4–6 mice/group). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus the indicated groups