Fig. 3

H151 administration reduced CD68 expression in microglia after stroke. A Representative immunofluorescent micrographs of IBA1 and CD68 after sham treatment or at different time points after stroke injury. B The number of IBA1-positive cells in each FOV in sham or PT group. C The percentage of CD68 positive area in each FOV. The results were relative to sham group. D The MFI of CD68 after sham or injury. E The number of CD68- and IBA1-double positive cells after sham or injury. n = 10–12 FOV from 3 mice. F Representative images of IBA1 and CD68 after administration of H151 or vehicle in sham-treated or PT-injured mice. Boxed regions were enlarged for analyzing colocalization. G–J The number of IBA1-positive cells (G), the percentage of CD68 positive area (H), CD68 MFI (I), and CD68/IBA1-double positive cells were quantified after H151 or vehicle administration at 7 days after sham or stroke. n = 8–9 FOV from 3 mice. Scale bar = 20 μm. Data were presented as mean ± SEM. **P < 0.01, ***P < 0.001