Fig. 4

STING inhibition affected the morphological features of microglia and reduced the levels of inflammatory cytokines. A Representative skeletonization of IBA1-labelled microglia. Scale bar = 5 μm. B–E Sholl analysis at 7 days after surgery for intersection numbers (B), endpoints (C), IBA1-positive area (D), and mean process length (E) per cell after consecutive administration of vehicle or H151. n = 17 cells from 3 mice per condition. Each dot represented an analyzed cell. F–I mRNA levels of Nlrp3 (F), Caspase1 (G), Il1β (H), and Tnfα (I) were detected by qRT-PCR at 7 days after surgery. n = 4–6 mice per condition. Each dot represented an individual mouse. Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001