Fig. 5

STING inhibition by H151 suppressed microglial phagocytosis of synapses after stroke. A Representative micrographs and 3D reconstructions of IBA1 and PSD95 at 7 days after surgery. B Quantitative data of PSD95 puncta number in IBA1-positive microglia. C Representative micrographs and 3D reconstructions of IBA1 and SYP after vehicle or H151 treatment. D Quantification of SYP puncta number in IBA1-positive microglia. E Representative images of SYP and PSD95. Boxed regions were enlarged for analyzing colocalization. F Relative SYP puncta number. G Relative puncta number of PSD95. H The quantification of SYP/PSD95 colocalized puncta number. The results were relative to sham + vehicle group. In (A–D), n = 23–40 cells from 3 mice per condition. Each dot represented an analyzed cell. In (E–H), n = 6–10 FOV from 3 mice per condition. Each dot represented a FOV. Scale bar = 5 μm. Data were presented as mean ± SEM. **P < 0.01, ***P < 0.001