Fig. 6

Genetically deleting STING inhibited microglial phagocytosis of synaptic elements after stroke. A The protein expression levels of STING in wildtype mice and STING-knockout mice. B Representative 3D reconstructions of IBA1 and synaptic elements, along with representative images of SYP and PSD95 at 7 days after surgery. C and D The quantification of SYP puncta number (C) and PSD95 puncta number (D) in IBA1-positive microglia. E The quantification of SYP/PSD95 colocalized puncta number. The results were relative to sham-treated wildtype mice. In (C, D), n = 11–74 cells from 3 mice per condition. Each dot represented an analyzed cell. In (E), n = 9–10 FOV from 3 mice per condition. Each dot represented a FOV. Scale bar = 5 μm. Data were presented as mean ± SEM. *P < 0.05, ***P < 0.001