Fig. 1

Immunohistochemical analysis of lymphoid cells in the brains of ⍺-synuclein transgenic mice treated with anti-CD1d. (A) Representative bright field light microscopy images from the neocortex, hippocampus and striatum of non-tg and ⍺-syn tg mice treated with IgG control or anti-CD1d and immunostained with an antibody against CD3 (general T cell marker). (B-D) Computer based image analysis showing comparable numbers of CD3 positive cell numbers in the neocortex (B), hippocampus (C) and striatum (D) of non-tg and ⍺-syn tg treated mice. (E) Representative bright field light microscopy images from the neocortex, hippocampus and striatum of non-tg and ⍺-syn tg mice immunostained with an antibody against CD4 (helper T cell marker). (F-H) Computer based image analysis showing increase numbers of CD4 positive cell in the brains of IgG injected ⍺-syn tg mice and lower levels in CD1d treated mice. (I) Representative bright field light microscopy images from the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice immunostained with an antibody against CD8 (cytotoxic T cell marker). (J-L) Computer based image analysis showing increase of CD8 positive cells in non-tg and ⍺-syn tg mice treated with anti-CD1d. Scale bar = 20 μm (high magnification). n = 4 mice per group. Statistical significance determined by two-way ANOVA with post-hoc Tukey; *, p ≤ 0.05; **, p ≤ 0.01