Fig. 7

Immunochemical analysis of ⍺-synuclein accumulation in the brains of ⍺-synuclein transgenic mice treated with anti-CD1d. (A) Representative bright field light microscopy images from the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d immunostained with an antibody against total ⍺-syn. (B–D) Computer based image analysis showing comparable levels of total ⍺-syn immunoreactivity in the neocortex, hippocampus, and striatum of IgG or anti-CD1d treated ⍺-syn tg mice. (E) Representative bright field light microscopy images from the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d immunostained with an antibody against p129 ⍺-syn. (F–H) Computer based image analysis showing similar levels of p129 ⍺-syn immunoreactivity in the neocortex, hippocampus, and striatum in IgG or anti-CD1d treated ⍺-syn tg mice. (I) Representative western blot analysis of brain homogenates from cytosolic and particulate fractions of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d. (J, K) Computer based image analysis showing slight reduction in total ⍺-syn in the cytosolic fraction of ⍺-syn tg mice treated with anti-CD1d but not in the particulate fraction. Scale bar = 30 μm. n = 4 mice per group. Statistical significance determined by two-way ANOVA with post-hoc Tukey; *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001