Fig. 1
Preparation and characteristics of Homer1a+ EVs. a Schematic diagram of A2 astrocyte induction. b Flow cytometry detection of the marker S100A10/C3 of A1/A2 astrocytes. c Schematic diagram of Homer1a+ EVs extraction. d Fluorescence of A2 astrocytes transfected with Homer1a-pWPI plasmid. e Size distribution and representative TEM images of EVs and Homer1a+ EVs. f The concentration of EVs and Homer1a+ EVs measured by nanoflow. g Western blotting analysis of EV-associated markers (CD9, CD63, CD81 and TSG101) in EVs and Homer1a+ EVs. h ELISA analysis of Homer1a in EVs from different treated cells [F (3, 8) = 147.7, P < 0.0001]. i WB analysis of Homer1a in EVs from different treated cells. j Quantification of result in panel i [F (3, 8) = 250.8, P < 0.0001]. k Homer1a concentration was detected by ELISA analysis at different time points under different conditions, including put in − 80 °C or 37° for 7 days. [Finteraction(12, 42) = 2.784, P = 0.0071]. l Comparison of the stability of Homer1a+ EVs and free Homer1a protein in pH 5.5 solution for 12 h. [Finteraction(1, 8) = 32.00, P = 0.0005]. The data were analyzed using one-way (h, j) or two-way (k, l) analysis of variance and all data are expressed as the mean ± standard deviation. ***P < 0.001 and ****P < 0.0001 represents a statistically significant difference between the two groups. ns: no statistical difference. The blots are representative of other replicates in those groups