Fig. 2
Homer1a+ EVs has therapeutic effect on ICH GFAPCreHomer1fl/−Homer1a± mice. a Schematic diagram of Homer1a conditional knockout Mouse model (C57BL/6 J) by CRISPR/Cas-mediated genome engineering. b Schematic diagram of GFAPCreHomer1fl/−Homer1a± mice injected with Homer1a+ EVs. c Detection of TNF-α level in brain tissue of each group by ELSIA [F (3, 8) = 111.3, P < 0.0001]. d Detection of IL-1β level in brain tissue of each group by ELSIA [F (3, 8) = 111.1, P < 0.0001]. e Longa scores of mice in different groups [F (3, 36) = 47.47, P < 0.0001]. f Survival curve of mice in each group (n = 10/group) [log-rank (Mantel–Cox) test: Chi-square = 10.40; df = 3; P = 0.0154]. g Representative photographs of HE staining of brain tissue in each group. h Quantification of result in panel g [F (3, 8) = 179.3, P < 0.0001]. i Representative photographs of Nissl staining of brain tissue in each group. j Quantification of result in panel i [F (3, 8) = 118.2, P < 0.0001]. k Representative photographs of TUNEL staining of brain tissue in each group. l Quantification of result in panel k [F (3, 8) = 131.0, P < 0.0001]. m Representative photographs of flow cytometry of primary neuron in each group. The data were analyzed using one-way analysis of variance and all data are expressed as the mean ± standard deviation. *P < 0.05, **P < 0.01 ***P < 0.001 and ****P < 0.0001 represents a statistically significant difference between the two groups. ns: no statistical difference