Fig. 3

Transcription and translation levels of C3 and S100A10 in primary astrocytes and mice ICH model after Homer1a⁺ EVs treatment. a Protein expression and quantification of C3 and S100A10 in brain tissues after Homer1a⁺ EVs treatment [C3: F (3, 8) = 249.8, P < 0.0001; S100A10: F (3, 8) = 311.3, P < 0.0001]. b mRNA level of C3 [F (3, 8) = 32.8, P < 0.0001)]. c mRNA level of S100A10 [F (3, 8) = 170.4, P < 0.0001)]. d Representative photographs of C3 and GFAP co-staining of frozen sections of brain tissue in different group. e Representative photographs of S100A10 and GFAP co-staining of frozen sections of brain tissue in different group. f Quantification of result in panel d [F (3, 8) = 54.9, P < 0.0001)]. g Quantification of result in panel e [F (3, 8) = 97.48, P < 0.0001)]. h Protein expression and quantification of C3 and S100A10 in primary neurons ICH models after Homer1a⁺ EVs treatment [C3: F (3, 8) = 113.1, P < 0.0001; S100A10: F (3, 8) = 63.67, P < 0.0001]. i mRNA level of C3 [F (3, 8) = 65.35, P < 0.0001)]. j mRNA level of S100A10 [F (3, 8) = 90.73, P < 0.0001)]. k Representative photographs of flow cytometry of primary astrocyte in each group. The data were analyzed using one-way analysis of variance and all data are expressed as the mean ± standard deviation. *P < 0.05, **P < 0.01 ***P < 0.001 and ****P < 0.0001 represents a statistically significant difference between the two groups. ns: no statistical difference. The blots are representative of other replicates in those groups