Fig. 4

RAGE/NF-κB/IL-17 signaling pathway was regulated by Homer1a⁺ EVs in vivo and in vitro. a Differential expression protein between cells and EVs. b KEGG analysis of 138 proteins detected only in EVs. c The expression level of RAGE/NF-κB/IL-17 signaling pathway related proteins was detected by WB in vivo. d The expression level of RAGE/NF-κB/IL-17 signaling pathway related proteins was detected by WB in vitro. e Detection of IL-17A level in vivo by ELSIA [F (3, 8) = 13.02, P = 0.0019]. f Detection of IL-17A level in vitro by ELSIA [F (3, 8) = 22.30, P = 0.0003]. g Representative photographs of IL-17AR and RAGE co-staining of frozen sections of brain tissue in different group. h Quantification of IL-17AR⁺ cells in panel g [F (3, 8) = 91.74, P < 0.0001)]. i Quantification of RAGE⁺ cells in panel g [F (3, 8) = 84.96, P < 0.0001)]. The data were analyzed using one-way analysis of variance and all data are expressed as the mean ± standard deviation. *P < 0.05, **P < 0.01 ***P < 0.001 and ****P < 0.0001 represents a statistically significant difference between the two groups. ns: no statistical difference. The blots are representative of other replicates in those groups