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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Fascin-1 limits myosin activity in microglia to control mechanical characterization of the injured spinal cord

Fig. 6

Physiologic changes in substrate stiffness alter Cx43 expression in astrocytes in vitro. (A) Experimental schematic diagram of astrocytes cultured on the substrate with 200 and 700 Pa. (B, C) Typical immunofluorescence images for the astrocyte marker GFAP (green, B) and vimentin (green, C) in different stiffness. Scale bar: 20 μm. (D) Quantitative analysis of the relative GFAP and vimentin intensity. (E) Typical immunofluorescence images for the Cx43 (red) in different stiffness. (F) Quantitative analysis of the relative Cx43 intensity. n = 6 independent cell cultures in (D) and (F). ****P < 0.0001 by Student’s t test

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Journal of Neuroinflammation

ISSN: 1742-2094