Fig. 4

Phagocytic assessment of Iba1⁺ cells in sMPTP mice. Animals were sacrificed at 10 days after the first MPTP injection. (A) Motor behavior was evaluated in the rotarod, bar and pole test. (B and C) Representative images of a single plane and the corresponding orthogonal projection of the stack of TH/Iba1 double immunofluorescence in (B) the striatum and (C) the SNpc of control and sMPTP mice. Quantitation of TH⁺ volume and percentage of TH⁺ signal in Iba1⁺ cells. (D) Quantitation of the number of ramified, hypertrophic and bushy Iba1⁺ cells in the striatum and SNpc from control and sMPTP mice. Data represent the mean ± 95% CI from 7 animals per group. Statistical analysis: (A, B and C) t-test with Welch correction when variances differed significantly, (D) 2-way ANOVA followed by Bonferroni post hoc test. Statistical significance in, (*) cell number, (^#) cell morphology. *^/#p < 0.05, **^/##p < 0.01, ***^/###p < 0.001. Magnification bars: 10 μm