Fig. 5

Phagocytic assessment of Iba1⁺ cells in cMPTP mice. Animals were sacrificed at 5 weeks after the first MPTP injection. (A) Motor behavior was evaluated in the rotarod, bar and pole test. (B and C) Representative images of a single plane and the corresponding orthogonal projection of the stack of TH/Iba1 double immunofluorescence in (B) the striatum and (C) the SNpc of control and cMPTP mice. Quantitation of TH⁺ volume and percentage of TH⁺ signal in Iba1⁺ cells. (D) Quantitation of the number of ramified, hypertrophic and bushy Iba1⁺ cells in the striatum and SNpc from control and cMPTP mice. Data represent the mean ± 95% CI from 7 animals per group. Statistical analysis: (A, B and C) t-test with Welch correction when variances differed significantly, (D) 2-way ANOVA followed by Bonferroni post hoc test. Statistical significance in (^#) cell morphology. ^#p < 0.05. Magnification bars: 10 μm