Fig. 6

Phagocytic assessment of Iba1⁺ cells in ecMPTP mice. Animals were sacrificed at 10 days after the first MPTP injection following the cMPTP intoxication pattern. (A) Motor behavior was evaluated in the rotarod, bar and pole test. (B and C) Representative images of a single plane and the corresponding orthogonal projection of the stack of TH/Iba1 double immunofluorescence in (B) the striatum and (C) the SNpc of control and ecMPTP mice. Quantitation of TH⁺ volume and percentage of TH⁺ signal in Iba1⁺ cells. (D) Quantitation of the number of ramified, hypertrophic and bushy Iba1⁺ cells in the striatum and SNpc from control and ecMPTP mice. Data represent the mean ± 95% CI from 7 animals per group. Statistical analysis: (A) Mann-Whitney test for the rotarod and bar test, t-test for the pole test; (B and C) t-test, (D) 2-way ANOVA followed by Bonferroni post hoc test. Statistical significance in, (*) cell number, (^#) cell morphology. *^/#p < 0.05, **^/##p < 0.01. Magnification bars: 10 μm