Fig. 7
From: Nigrostriatal degeneration determines dynamics of glial inflammatory and phagocytic activity
Phagocytic assessment of astrocytes in MPTP mice. Animals were sacrificed at 10 days (sMPTP and ecMPTP) or at 5 weeks (cMPTP) after the first MPTP injection. (A) Representative images of a single plane and the corresponding orthogonal projection of the stack of TH/GFAP double immunofluorescence in the striatum of sMPTP, ecMPTP and cMPTP mice. Quantitation of GFAP+ volume and percentage of TH+ signal in GFAP+ cells. Dashed line corresponds to the mean of GFAP+ volume in control animals. (B) Representative images of a single plane and the corresponding orthogonal projection of the stack of TH/GFAP double immunofluorescence in the SNpc of sMPTP, ecMPTP and cMPTP mice. Quantitation of GFAP+ volume. Dashed line corresponds to the mean of GFAP+ volume in control animals. Data represent the mean ± 95% CI from 7–8 animals per group. Statistical analysis: (A) One-way ANOVA test. **p < 0.01, ***p < 0.001. Magnification bars: 10 μm