Fig. 2

OA alleviates early brain injury and neuronal damage after SAH in vitro and in vivo.A: Schematic representation illustrates the experimental procedure for simulating SAH in mice and HT22 cells. B: Cell viability results show that OA addition dose-responsively diminished neuronal damage, with 5 µM OA selected as the test concentration (n = 5). C: Representative views of Dead/Live staining following OA treatment. Scale bar = 100 μm. D: Neurological scores of mice in the three groups following SAH (n = 15). E: Brain water content comparison among the three groups after SAH (n = 5; ^*p < 0.01 vs. Sham, ^#p < 0.05 vs. SAH; t-test; mean ± SD). F: TUNEL assay shows that OA attenuates neuronal death in the hippocampus following SAH. Scale bar = 50 μm