Fig. 5

Effects of Mas activation with AVE0991 on neutrophil infiltration and inflammatory cytokine release after ICH. A Representative images of immunofuorescence staining of MPO (green) and IL-1β (green) in the perihematomal area at 3 days after ICH. Scale bar = 50 μm. B Regions of interest areas in the perihematomal region. C Quantitative analyses of MPO and IL-1β-positive cells in the perihematomal area at 3 days after ICH, n = 6/group. D, E Representative Western blot bands and quantitative analyses of MPO and IL-1β protein levels at 3 days after ICH. F, G mRNA levels were evaluated including CD16, CD32, IL-1β, iNOS, IL-6, and CD11b. Pro-inflammatory cytokines-associated mRNA levels were evaluated including IL-1β and IL6. Anti-inflammatory cytokines-associated mRNA levels were evaluated including IL-4 and IL10. Data were represented as mean ± SD. ***P < 0.001 vs. Sham group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. ICH + Vehicle group. n = 6/group