Fig. 3
From: Alpha-synuclein inclusion responsive microglia are resistant to CSF1R inhibition
CSF1R inhibition does not impact pSyn aggregation or early loss of TH-immunoreactivity in the SNpc. A Phosphorylated alpha synuclein (pSyn) inclusions in the ipsilateral substantia nigra pars compacta (SNpc) 2 months post alpha synuclein preformed fibril (α-syn PFF) injection in both Pexidartinib (PLX3397B) and control fed rats. B Quantification of pSyn immunoreactive (pSynir) neurons in the ipsilateral SNpc 2 months after α-syn PFF injection in control and PLX3397B rats. PLX3397B treatment had no impact on the number of pSynir neurons within the SNpc. C Tyrosine hydroxylase immunoreactive (THir) neurons in the SNpc of α-syn PFF and control phosphate buffered saline (PBS) injected rats, with and without PLX3397B treatment. D Quantification of THir neurons in the SNpc 2 months following injection. PFF injected rats possessed significantly fewer THir neurons in the ipsilateral SNpc as compared to the ipsilateral SNpc of PBS injected rats. No differences in THir neurons were observed due to PLX3397B treatment. Values represent the mean ± SEM. Black outline = no PLX3397B; green outline = PLX3397B; *p = 0.0330; **p = 0.0058. Scale bars in A and C are 100 µm