Fig. 7
From: Alpha-synuclein inclusion responsive microglia are resistant to CSF1R inhibition
CSF1R inhibition does not impact degeneration of nigrostriatal dopamine neurons following α-synuclein preformed fibril injection. A Phosphorylated α-syn (pSyn) inclusions in the ipsilateral substantia nigra pars compacta (SNpc) 6 months post alpha-synuclein preformed fibril (α-syn PFF) injection in both Pexidartinib (PLX3397B) and control fed rats. B Quantification of pSyn immunoreactive (pSynir) neurons in the ipsilateral SNpc in rats 6 months after α-syn PFF injection. Significantly fewer pSynir SNpc neurons are observed in α-syn PFF rats fed PLX3397B. C Tyrosine hydroxylase immunoreactive (THir) neurons in the SNpc of α-syn PFF and control phosphate buffered saline (PBS) injected rats, with and without 6 months of PLX3397B treatment. D Quantification of THir neurons in the SNpc 6 months following surgery. α-syn PFF injection resulted in significant loss of THir SNpc neurons in both PLX3379B and control fed rats. Values represent the mean ± SEM. ****p < 0.0001 *p < 0.05. PLX3397B = green outline, no PLX3397B = black outline. Scale bars in A and C are 100 µm