Table 1 Oligonucleotide primers used for PCR, and the corresponding restriction endonucleases used for product confirmation.
From: Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs
Gene | Sequence (5' → 3') | Fragment size (introns) | Genbank accession No | Restriction enzymes used and the expected sizes of digestion products (bp) |
|---|---|---|---|---|
C1 inh-F | GTT GGG GGA TGC TTT GGT AGA TTT C | 332 | M13690 | Sau 3AI (246, 86) |
C1 inh-R | TTA GGA CTC TGG GGC TGC TGC TGT A | (2 introns) | Â | Â |
CD59-F | CTG CTG CTC GTC CTG GCT GTC TTC T | 280 | M34671 | Pst I (233, 47) |
CD59-R | TCC CAC CAT TTT CAA GCT GTT CGT T | (2 introns) | Â | Â |
MCP-F | CAA TTC AGT GTG GAG TCG TGC TGC | 265 | Y00651 | Sau 3AI (193, 72) |
MCP-R | TGA GGC ACT GGA CGC TGG AGA T | (unknown) | Â | Â |
DAF-F | GTA CTG TGA ATA ATG ATG AAG GAG | 364 | M30142 | Hae III (330, 34) |
DAF-R | TCT TAA CTC TTC TTT GGC TAA GTC | (unknown) | Â | Â |
G3PDH-F | CCA TGT TCG TCA TGG GTG TGA ACC A | 251 | X01677 | Dde I (168, 83) |
G3PDH-R | GCC AGT AGA GGC AGG GAT GAT GTT C | (2 introns) | Â | Â |