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Table 1 Oligonucleotide primers used for PCR, and the corresponding restriction endonucleases used for product confirmation.

From: Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs

Gene Sequence (5' → 3') Fragment size (introns) Genbank accession No Restriction enzymes used and the expected sizes of digestion products (bp)
C1 inh-F GTT GGG GGA TGC TTT GGT AGA TTT C 332 M13690 Sau 3AI (246, 86)
C1 inh-R TTA GGA CTC TGG GGC TGC TGC TGT A (2 introns)   
CD59-F CTG CTG CTC GTC CTG GCT GTC TTC T 280 M34671 Pst I (233, 47)
CD59-R TCC CAC CAT TTT CAA GCT GTT CGT T (2 introns)   
MCP-F CAA TTC AGT GTG GAG TCG TGC TGC 265 Y00651 Sau 3AI (193, 72)
MCP-R TGA GGC ACT GGA CGC TGG AGA T (unknown)   
DAF-F GTA CTG TGA ATA ATG ATG AAG GAG 364 M30142 Hae III (330, 34)
DAF-R TCT TAA CTC TTC TTT GGC TAA GTC (unknown)   
G3PDH-F CCA TGT TCG TCA TGG GTG TGA ACC A 251 X01677 Dde I (168, 83)
G3PDH-R GCC AGT AGA GGC AGG GAT GAT GTT C (2 introns)