Figure 4

Specificity of primary iNOS antibody confirmed by immunohistochemistry. GFAP immunoreactivity was detected in both wild type (a) and iNOS knockout (b) mouse astrocyte monolayer cultures confirming the presence of astrocytes. iNOS immunoreactivity was not detected in wild type (c) or iNOS knockout (d) cultures treated with vehicle alone. In wild type cultures treated with LPS+IFN-γ, iNOS immunoreactivity increased (e). However iNOS was not detected in iNOS knockout cultures treated with LPS+IFN-γ (f). Bar= 20 μm.