Figure 7

Adenylate cyclase activation mediates LPS induced NO production. (A) Microglia were incubated with LPS (1 ng/ml) alone or with NE (1 μM), and in the presence of the AC inhibitor SQ 22536 (SQ); or with LPS in presence of the irreversible cell permeable AC inhibitor MDL-12,330A (MDL). *** and **, P < 0.001 and 0.01 versus LPS alone. (B) Total cytosolic RNA was prepared from control microglia, or microglia incubated for 24 h with 1 ng/ml of LPS in presence of 200 μM SQ22536, or 10 μM NE or both SQ and NE and used for Q-PCR analysis of NOS2 mRNA. Data are expressed as percentage of LPS (100%). ***, P < 0.001 versus LPS alone; one-way ANOVA.