Neuronal expression of IL-1β in the right hemisphere exposed to lipopolysaccharide (LPS) + hypoxia-ischemia (HI). Mean ± standard error of the mean (SEM) (pg/mg) of IL-1β (A) and TNF-α (B). Titrations were done by ELISA on total protein extracts from the right cerebral hemisphere exposed to LPS plus HI for 4 h to 24 h compared to control (Ctl). (C) Immunofluorescent staining of neuronal nuclei (NeuN) + IL-1β + cells in the fronto-parietal neocortex (D). Mean ± SEM (%) of neural cells expressing Il-1β. (E) Immunohistochemical staining of IL-1β expression on neurons (arrows) from fronto-parietal neocortex and caudate putamen at 4 h post-LPS + HI compared to Ctl. Scale bar = 50 μm (4 to 6 pups per experimental condition). Analysis of variance with Newman-Keuls post-test; *
P ≤ 0.05, **
P ≤ 0.01. h hour, F-P Fronto-parietal, WM White matter, CPu Caudate putamen, Iba1 Ionized calcium-binding adapter molecule 1, GFAP Glial fibrillary acidic protein.