Figure 4

Microglia/macrophage activation in the spinal area adjacent to the lesion site but not around the distal hematoma at 3 days post injury. (A) Montage picture of longitudinal spinal cord section for IBa-1 immunolabeling, showing lesion site (indicated by letter L) and hematoma (indicated by letter H) at 3 days post injury. (B) Amplified picture of the box adjacent to the lesion site, showing many cells are labeled with both IBa-1 (red), the marker of microglia, and ED-1(green), the marker of activated microglia. (C) Amplified picture of the box adjacent to the far-away hematoma in which only IBa-1 positive cells with thin processes could be seen. Bar = 2 mm in (A). Bar = 50 μm in (B) and (C).