Figure 2
From: Exercise reduces activation of microglia isolated from hippocampus and brain of aged mice
Proportion of brain microglia positive for MHC II and CD86 in Experiment 1. (A) Average proportion of microglia isolated from the brains of running-wheel mice and control mice that were positive for MHC II. Aged males (n=7), but not aged females (n=7) or adult mice (males n=9, females n=9), showed an increase in the proportion of MHC II positive microglia from running-wheel access. (B) Average proportion of microglia isolated from the brains of running-wheel mice and control adult and aged mice that were positive for CD86. Aged males, but not females, showed a significant reduction in the proportion of CD86 positive microglia from running. Bars represent means ± SEM. * indicates a significant difference from age- and sex-matched control mice. # indicates a significant difference from age-matched female mice. (C) Histogram of MHC II signal intensity from non-stained control sample; M1 indicates the gate for the MHC II positive cells. (D) Histogram of CD86 signal intensity from non-stained control sample; M1 indicates the gate. (E) Histogram of representative hippocampal sample labeled for MHC II. (F) Histogram of representative hippocampal sample labeled for CD86.