Figure 4

NSPC ^IL-10 and NSPCs inhibit proliferation and cytokine production by T-cells. (a) C57BL/6 spleen cells were polyclonal-activated with ConA in the presence of NSPC^IL-10 (black squares), NSPCs (open diamonds) or neurobasal medium (open circles) as indicated. Proliferation was determined by ³H-thymidine incorporation. IFN-γ and IL-2 production was assessed by ELISA. *P <0.05. Mean values are expressed with SD from three independent experiments. (b) Frequency of CD25⁺ cells of CD4 T-cells of MOG35-55-stimulated 2D2 spleen cells or ConA-stimulated spleens cells after cultivation with NSPC^IL-10, NSPCs or neurobasal medium. Analysis was performed by flow cytometry. *P <0.05. NSPC, neural stem/progenitor cell; ConA, concanavalin A; ELISA enzyme-linked immunosorbent assay; IFN, interferon; IL, interleukin; MOG35-55, myelin oligodendrocyte glycoprotein aa 35–55.