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Table 2 Immunocytochemistry: primary antibodies

From: Differential expression of major histocompatibility complex class I in developmental glioneuronal lesions

Antigen Primary antibody Source Dilution
Glial fibrillary acidic protein (GFAP) Polyclonal rabbit DAKO, Glostrup, Denmark 1:4,000
Neuronal nuclear protein (NeuN) Mouse clone MAB377 Chemicon, Temecula, CA, USA 1:2,000
HLA-DP, DQ, DR (MHC-II) Mouse clone CR3/43 DAKO, Glostrup, Denmark 1:1000
HLA-A, B, C (MHC-I) Mouse clone HC-10 Gift from Prof. J. Neefjes, NKI, the Netherlands 1:200
Vimentin Mouse clone V9 DAKO, Glostrup, Denmark 1:400
Neurofilament (NF) Mouse clone SMI311 Sternberger Monoclonals, Lutherville, MD, USA 1:1,000
Microtubule associated protein (MAP2) Mouse clone HM2 Sigma,St. Louis, IL, USA 1:100
Microtubule associated protein (MAP2) Polyclonal rabbit Millipore, Billerica, MA, USA 1:500
Phospho-S6 ribosomal protein (pS6) Polyclonal rabbit (Ser235/236; pS6) Cell Signaling Technology, Beverly, MA, USA; 1:50
CD68 Mouse clone PG-M1 DAKO, Glostrup, Denmark 1:200
CD31 Mouse clone JC70A DAKO, Glostrup, Denmark 1:10
Albumin Rabbit polyclonal DAKO, Glostrup, Denmark 1:20,000
Granzyme B (GrB) Mouse clone GrB-7 Monosan, Uden, The Netherlands 1:100