Table 2 Immunocytochemistry: primary antibodies
Antigen | Primary antibody | Source | Dilution |
|---|---|---|---|
Glial fibrillary acidic protein (GFAP) | Polyclonal rabbit | DAKO, Glostrup, Denmark | 1:4,000 |
Neuronal nuclear protein (NeuN) | Mouse clone MAB377 | Chemicon, Temecula, CA, USA | 1:2,000 |
HLA-DP, DQ, DR (MHC-II) | Mouse clone CR3/43 | DAKO, Glostrup, Denmark | 1:1000 |
HLA-A, B, C (MHC-I) | Mouse clone HC-10 | Gift from Prof. J. Neefjes, NKI, the Netherlands | 1:200 |
Vimentin | Mouse clone V9 | DAKO, Glostrup, Denmark | 1:400 |
Neurofilament (NF) | Mouse clone SMI311 | Sternberger Monoclonals, Lutherville, MD, USA | 1:1,000 |
Microtubule associated protein (MAP2) | Mouse clone HM2 | Sigma,St. Louis, IL, USA | 1:100 |
Microtubule associated protein (MAP2) | Polyclonal rabbit | Millipore, Billerica, MA, USA | 1:500 |
Phospho-S6 ribosomal protein (pS6) | Polyclonal rabbit (Ser235/236; pS6) | Cell Signaling Technology, Beverly, MA, USA; | 1:50 |
CD68 | Mouse clone PG-M1 | DAKO, Glostrup, Denmark | 1:200 |
CD31 | Mouse clone JC70A | DAKO, Glostrup, Denmark | 1:10 |
Albumin | Rabbit polyclonal | DAKO, Glostrup, Denmark | 1:20,000 |
Granzyme B (GrB) | Mouse clone GrB-7 | Monosan, Uden, The Netherlands | 1:100 |