mice deficient for the urokinase plasminogen activator (uPA
), or the urokinase plasminogen activator receptor (uPAR
) show reduced T-cell reactivity to myelin oligodendrocyte glycoprotein (MOG)
and reduced antigen presentation. (A) Proliferation of lymphocyte from all the tested groups was checked by [3H] thymidine incorporation in the presence of MOG35-55. (B). The effect of uPA and uPAR deficiency on T helper 1 (Th1) cytokine secretion. Lymphocytes from uPA−/−, uPAR−/− and WT mice were stimulated with MOG35-55, the media were collected after 24 hours, and the secreted (B) interferon (IFN)-γ and (C) tumor necrosis factor (TNF)-α were measured by ELISA. (A–C) Results are expressed as mean ± standard error (SE) of three separate experiments (*P<0.05) (D) Effect of uPA and uPAR deficiency on antigen presentation measured by MOG35-55 specific T-cell proliferation. Lymphocyte proliferation was checked by [3H] thymidine incorporation using APCs from knockout (KO) or WT mice in the presence of MOG35-55. Results are expressed as the mean ± SE of four separate experiments (*P<0.05).