Figure 3
From: A novel autoantibody against ephrin type B receptor 2 in acute necrotizing encephalopathy
Confirmation of anti-EphB2 activity of V10-5 IgG. (A) Nonpermeabilized human umbilical vein endothelial cells (HUVECs) and human brain microvascular cells (HBMECs) were stained with isotype control or anti-EphB2 antibody followed by secondary antibody, and analyzed by flow cytometry. (B) Inhibition tests of binding activities to HBMECs were performed using V10-5 IgG with soluble EphB2 at the indicated concentrations. Control indicates binding activity of IgG from healthy donor to HBMECs. (C) EphB2-expressing or non-expressing YB2/0 cells were stained with 1:10 diluted V10-5 serum followed by secondary antibody against human IgG1, IgG2, IgG3, IgG4, or IgM, and analyzed by flow cytometry.