Astrocytes acquire resistance to iron-dependent oxidative stress upon proinflammatory activation

Table 1 mRNA expression of selected genes under activation conditions

Gene	2CK vs. C	MCM[+] vs. C	MCM[+] vs. C (Röhl et al., 2008)
Rbp1	2,300	1,697	490
Sod2	1,511	2,322	2,200
Ass	1,422	384	135
Txnrd1	173	133	145
Gss	141	126	93
Prdx5	137	241	120
Prdx6	136	105	63
Cat	132	86	63
Gstp1	123	111	220
Hsbp1	119	92	45
Sod1	110	93	96
Mt1a	106	475	67
Nme1	104	91	80
Gstm3	102	92	62
G6pdx	101	91	100
Nme2	94	104	90
Gsr	90	104	135
Gstm1	90	116	88
Gpx1	81	65	60
Gstm2	75	120	120
Gclc	53	42	73

RNA was extracted from resting astrocytes (C, control) or astrocytes activated for 24 h with either proinflammatory cytokines (CK: 10 ng/ml IL-1β and 30 ng/ml TNFα) or conditioned medium from LPS-activated microglia (MCM[+]). The gene expression levels were determined through the Illumina BeadArray technology using RatRef-12 Expression Beadchips. The values corresponding to each gene expressed by activated astrocytes represent the percent with respect to the values of resting astrocytes. Values of the changes in gene expression relative to activated astrocytes in Röhl’s work were extrapolated from the analysis shown in the corresponding paper [23].

ISSN: 1742-2094