Figure 5

Effects of genetic deletion and blockade of FP receptor on the ionized calcium-binding adapter protein 1 (Iba1) expression after controlled cortical injury (CCI). (A and B) Representative photographs of Iba1 immunohistochemistry on hippocampal brain sections from sham- and CCI-injured WT (A) and FP^-/- (B) mice 48 hours after injury. Rectangular selections in the inserts denote magnified regions. (C) Bar graph represents quantitative analysis of Iba1 immunoreactivity in cortical penumbra, hippocampus, and thalamus in saline- and AL-8810- (10 mg/kg) treated WT and FP^-/- mice that underwent CCI or sham 48 hours and 10 days following injury. All data are normalized to the corresponding value of the contralateral side. Data are presented as mean ± SEM, *P < 0.05, **P < 0.01 versus saline-treated WT sham-operated animals, ^#P < 0.05, ^##P < 0.01, versus saline-treated WT CCI group, one-way ANOVA followed by Tukey's multiple comparison test (n = 3 to 7).