Table 1 Cellular expression of ADAMTS proteoglycanases in the physiological and pathological central nervous system
From: ADAMTS proteoglycanases in the physiological and pathological central nervous system
Cell type | ADAMTS-1 | ADAMTS-4 | ADAMTS-5 | ADAMTS-9 | ||
|---|---|---|---|---|---|---|
Astrocytes | In vitro: | Primary cerebral cultures (mouse) [34] | Yes (mRNA) | Yes (mRNA) | Yes (mRNA) | Yes (mRNA) |
Yes (mRNA/WB) | ||||||
Primary cerebral cultures (human) [23]: | Yes (mRNA/WB/ICC) | Yes (mRNA/WB/ICC) | Yes (mRNA/WB/ICC) | |||
- TNF-α treatment | ↑ mRNA | ↑ mRNA | stable mRNA | |||
stable protein | ↑ protein | stable protein | ||||
- IL1-β treatment | stable mRNA | stable mRNA | stable mRNA | |||
In vivo: | Brain white matter (human) [22] | Yes (IHC) | Yes (IHC) | Yes (IHC) | ||
Spinal cord white matter (rat) [21] | Yes (IHC) | Yes (IHC) | Yes (IHC) | |||
Injured brain, tMCAO (mouse) [33] | Yes (mRNA) | Yes (mRNA) | ||||
Injured cerebral cortex, tMCAO (rat) [32] | Yes (mRNA) | |||||
Injured spinal cord, SCI by contusion (mouse) [34] | Yes (IHC) | Yes (IHC) | Yes (IHC) | |||
Neurons | In vitro: | Primary cortical cultures (rat) [25] | Yes (WB) | |||
Primary cerebellar granule neurons cultures (rat) [24] | No (mRNA) | |||||
In vivo: | Hippocampus, dentate granule neurons and pyramidal neurons (rat) [16]: | |||||
- Physiological conditions | No (mRNA) | Yes (mRNA) | ||||
- Kainate-induced CNS excitoxicity | Yes (mRNA) | Yes (mRNA) | ||||
Injured cerebral cortex, tMCAO (rat) [32] | Yes (mRNA) | |||||
Injured spinal cord, SCI by contusion (mouse) [34] | No (IHC) | |||||
Microglia | In vitro: | Yes (mRNA/WB) | ||||
Monocytes / | In vitro: | Yes (mRNA) | Yes (mRNA/WB) | Yes (mRNA) | Yes (mRNA) | |
Macrophages | In vitro: | Yes (mRNA) | Yes (mRNA/WB) | Yes (mRNA) | Yes (mRNA) | |
↑ mRNA | ↑ mRNA/↓ mRNA | ↑ mRNA | ↑ mRNA | |||
↓ mRNA | stable/↑ mRNA | stable mRNA | ↑ mRNA | |||
- TNF-α treatment [37] | ↑ mRNA | slight ↑ mRNA | ||||
- IL1-β treatment [37] | slight ↑ mRNA | stable mRNA | slight ↑ mRNA |