Figure 6
From: Involvement of NT3 and P75NTRin photoreceptor degeneration following selective Müller cell ablation
Intravitreal injection of recombinant NT3 and blockage of P75NTRprotected photoreceptor degeneration. Intravitreal injection was performed in transgenic mice 3d and 6d after tamoxifen induced Müller cell ablation, with one eye receiving the testing reagent and the contralateral eye receiving balanced salt solution (BSS). Doses of injection: (1) NT3, 0.4 μg; (2) P75NTR rabbit polyclonal Ab (1:1 dilution) and (3) 0.4 μg NT3 + 1:1 dilution of P75NTR Ab. Eyes were enucleated 10d after TMX treatment for flat-mount staining using fluorescence-conjugated peanut-agglutinin (PNA). (A and B) PNA-labeled cone photoreceptor outer segments (green) and nuclear counterstaining in a control (Ctl) retina. (C-F) Images from transgenic (TG) mice showing changes in cone photoreceptor outer segments and protrusion of photoreceptor nuclei into the subretinal space (red, indicated by asterisks) in eyes receiving BSS (C), NT3 (D), P75NTR Ab (E) and a combination of NT3 and P75NTR Ab (F). (G) Quantitative analysis of PNA staining showed that intravitreal injection of NT3 and P75 Ab protected the loss of cone photoreceptor outer segments, with a combined treatment more effective than either alone. N = 9 − 11/group. Scale bars in A-F: 100 μm.