Table 1 Dilutions for the primary antibodies used for immunohistochemistry or immunocytochemistry
Antigen | Species, type | Dilution | Catalog number, manufacturer |
|---|---|---|---|
NF-IL6 | Goat polyclonal IgG | 1:250 | cat. sc-150-G; Santa Cruz Biotechnology, Santa Cruz, CA, USA |
NF-IL6 | Rabbit polyclonal IgG | 1:9000 | cat. sc-150; Santa Cruz Biotechnology, Santa Cruz, CA, USA |
STAT3 | Rabbit polyclonal IgG | 1:8000 | cat. sc-21876; Santa Cruz Biotechnology, Santa Cruz, CA, USA |
NFκB | Goat polyclonal IgG | 1:500 | cat. sc-372; Santa Cruz Biotechnology, Santa Cruz, CA, USA |
VWF | Sheep polyclonal IgG | 1:3000 | cat. SARTW-IG; Affinity Biologicals, Ancaster, Canada |
GFAP | Mouse monoclonal IgG | 1:2000 | cat. MAB3402; Millipore, Billerica, MA, USA |
CD68 (ED1) | Mouse monoclonal IgG | 1:1000 | cat. MCA341R; AbD Serotec, Oxford, United Kingdom |
CD163 (ED2) | Mouse monoclonal IgG | 1:500 | cat. MCA342R; AbD Serotec, Oxford, United Kingdom |
NOS1 | Rabbit polyclonal IgG | 1:500 | cat. sc-648; Santa Cruz Biotechnology, Santa Cruz, CA, USA |
ACTH (1–24) | Rabbit polyclonal IgG | 1:2000 | Gift from Dr. Blähser, Institute of Anatomy and Cytobiology, JLU Giessen, Germany (Blähser, 1988) |
Anti- S100 | Rabbit polyclonal IgG | 1:250 | cat. S2644 Sigma-Aldrich, Munich, Germany |
TNFα | Goat polyclonal IgG | 1:2000 | cat. AF-510-NA R&D Systems, MN, USA |