Figure 5

Immunohistochemical analysis of the effect of subacute LPS treatment on α-synuclein. PBS (2 μL/injection, bilaterally) or LPS (5 μg/2 μL/injection, bilaterally) was injected into the SNR daily for 5 consecutive days. (A) Representative photomicrographs are coronal sections of the SNC 30 days after the final PBS (upper) or LPS treatment (lower). Triple immunofluorescence staining was performed with α-synuclein (red) antibody, TH (green) antibody and DAPI (blue). Scale bars: 20 μm (B) Effect of LPS treatment on the gene expression of α-synuclein in the midbrain 30 days after the final LPS treatment. Values are expressed as percentages of those in the PBS-treated group and represented as mean ± SEM. *P < 0.05 compared with the PBS-treated group. DAPI, 4',6-diamidino-2-phenylindole; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; SEM, standard error of the mean; SNC, substantia nigra pars compact; SNR, substantia nigra pars reticulata; Τ Η , tyrosine hydroxylase.