Figure 5

TRIF and MyD88 signaling deficient mice have different Iba-1 immunoreactivity profiles following L5 SNL. At day 14 following L5 SNL the lumbar region of the spinal cord was harvested and incubated with antibodies against Iba-1 and GFAP. Both (A) Iba-1 and (B) GFAP immunoreactivity were consistently significantly elevated in the ipsilateral side of the dorsal horn compared to contralateral within each group. Data expressed as mean ± SEM (n = 3–5 sections per mouse, with 3–4 mice/group) and analyzed via 2-way ANOVA followed by Bonferroni post hoc test. The Iba-1 Trif ^lps2 and Myd88 ^-/- ipsilateral groups are statistically different from the C57BL/6 ipsilateral group (**P <0.01; ****P <0.0001). No significant difference was found among the contralateral groups. Representative stained images are presented from C57BL/6 mice with (C) Iba-1 and (D) GFAP, Myd88 ^-/- mice with (E) Iba-1 and (F) GFAP, and Trif ^lps2 mice with (G) Iba-1 and (H) GFAP, which support the quantified immunoreactivity results.