Figure 6

Hon and Mag inhibit cytokine-induced ROS production in BV-2 microglial cells. (A) Time course for ROS production induced by LPS or IFNγ. Cells were treated with LPS (100 ng/ml) or IFNγ (10 ng/ml) for the time indicated. ROS production was measured using CM-H2DCFDA as described in the text. Similarly, (B) Hon and (C) Mag (1 to 10 μM) were given 1 h prior to exposure to IFNγ (10 ng/ml) for 12 h. Results are expressed as the mean ± SEM (n = 3) and significant difference from the respective IFNγ-stimulated group was determined by one-way ANOVA followed by Dunnett’s tests, *P < 0.05; ** P < 0.01.