Figure 7

Cytokine levels induced after an inflammatory cocktail or IL-1β stress in primary tri-cultures. Cytokine (IL1-β, TNF-α, and IL-6) levels in cell lysates (A, B, C, respectively) and in culture medium (D, E, F, respectively) of tri-cultures pre-treated or not with 210 nM C16 and exposed or not to 20 μM Aβ42, and treated with a cocktail of IL-1β, TNF-α, and IL-6 (200 pg/mL of each cytokine) or with 200 pg/mL IL-1β alone in serum-free medium were analyzed by the 3-plex Luminex xMAP assay containing a mixture of specific beads for each cytokine as described in the Methods section. Cytokine levels in cell lysates and culture medium are expressed in pg/mg protein and pg/mL, respectively. Results are mean ± SEM for six experiments in duplicate. ^*** P <0.001 compared to respective control; ^# P <0.05, ^### P <0.001 compared to inflammatory cocktail; ^†† P <0.01, ^††† P <0.001 compared to IL-1β, ^ϵϵ P <0.01, ^ϵϵϵ P <0.001 compared to IL-1β with Aβ42 by one-way ANOVA with a Newman–Keuls multiple comparison test.