Figure 4

GluR4 mRNA and protein expression in activated mixed glial cultures. Quantitative real-time PCR (A, B) and western blotting (C) were performed to detect the AMPA subunit, GluR4, expressed in mixed glial cultures in the absence and presence of TNF-α (A) or LPS (B). Data are presented as fold change in gene expression relative to time-matched controls and standardised to 18 s. Protein expression is relative to β-actin + SEM of four independent experiments. * P <0.05.