Skip to main content
Figure 11 | Journal of Neuroinflammation

Figure 11

From: Cellular and temporal expression of NADPH oxidase (NOX) isotypes after brain injury

Figure 11

NOX inhibition reduces microglial activation and toxicity. BV2 microglial cells incubated with PMA or LPS showed an increase in ROS production (A). Pre-incubation with the NOX2 specific inhibitor gp91ds-tat (10 μM), or the NOX non-specific inhibitor DPI (10 μM), significantly reduced the ROS production induced by PMA and LPS, respectively (A). LPS also induced NO release from BV2 cells, which was inhibited by gp91-ds-tat (10 μM) and in a dose-dependent fashion by DPI (B). Co-culture of oligodendrocytes with LPS-stimulated BV2 cells for 24 hours resulted in a significant increase in oligodendrocyte cell death, as measured by LDH release (C). Pre-treatment of BV2 cells with DPI (10 μM) for 1 hour prior to LPS administration blocked this toxic effect. Bars represent mean ± SD. *P <0.05; **P <0.01; ***P <0.001.

Back to article page